Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acquired pyroglutamic acidosis due to long term dicloxacillin and paracetamol use BMJ Case Reports Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Pyroglutamic acidosis Deranged Physiology Mechanism of paracetamol toxicity NUS Faculty of Science NUS Faculty of Science