Full article: Paracetamol: overdose induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Paracetamol toxicity Deranged Physiology Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Paracetamol is one of the most commonly used analgesic and antipyretic drugs.